EMBRYONIC INDUCTIONS BY MICROSOMAL FRACTIONS SEPARATED WITH SODIUM DEOXYCHOLATE1)

Sodium/calcium exchange in smooth-muscle microsomal fractions.

The existence of Na+ -dependent Ca2+ transport was investigated in microsomal fractions from the longitudinal smooth muscle of the guinea-pig ileum and from the rat aorta, and its activity was compared with that of the plasmalemmal ATP-dependent Ca2+ pump previously identified in these preparations. The rate of Ca2+ release from plasmalemmal vesicles previously loaded with Ca2+ through the ATP-...

The sodium-plus-potassium ion-activated adenosine triphosphatase of cerebral microsomal fractions: treatment with disrupting agents.

1. The Na(+)-plus-K(+)-stimulated adenosine triphosphatase [(Na(+),K(+))-ATPase] of microsomal preparations from ox brain was inactivated or diminished in activity by exposure to 2-8m-urea. Similar concentrations of urea diminished the turbidity of the suspensions. 2. Low concentrations (about 2.5mm) of NaATP with the urea gave partial or complete protection of the ATPase, without altering the ...

Properties of alkaline-phosphatase fractions separated by starch-gel electrophoresis.

Studies of Human Ceruloplasmin Fractions Separated by Chromatography on Hydroxylapatite.

Recent physicochemical(l,2) and immunochemical(3) studies from our laboratory have shown that human ceruloplasmin is a labile protein the properties of which are readily modified by a variety of experimental conditions. Our previous data, however, indicated that crystalline ceruloplasmin was a single protein. This is at variance with results from other laboratories which have shown that chromat...

Glycerol 3-phosphate acylation by microsomal fractions from avocado mesocarp.

Triacylglycerol synthesis in plants occurs via the classical Kennedy pathway [1,2]. The quality and physical properties of the triaqlglycerol products are determined by the substrate specificities of the acyltransferases involved [2]. The initial step is catalysed by glycerol 3-phosphate acyltransferase and involves the acylation of the sn-1 position of lycerol 3-phosphate to form lysophosphati...